Correlation between expression of apoptosis-related gene pnas-2 and leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 738-742, 2007.
Article
in Zh
| WPRIM
| ID: wpr-276832
Responsible library:
WPRO
ABSTRACT
The study was purposed to explore the correlation between apoptosis-related gene pnas-2 and leukemia. The RT-PCR was performed to detect the expression levels of pnas-2 gene in NB4, K562, U937 cells before and after treatment with AS(4)S(4), and to analysis the expression change of pnas-2 gene in bone marrow cells from patients with acute leukemia before and after chemotherapy. The results showed that the expression of pnas-2 gene in arsenic sulfide treated NB4 cells was down regulated in time-dependent manner, but the same outcome in K562 and U937 cells after being treated with AS(4)S(4) was not found. The positive expression rate of pnas-2 in cells from untreated patients with acute leukemia was 100%, and was significantly higher than that in normal control group. After chemotherapy, the expression was negative in complete remission patients, whereas in no-remission patients there were no significant differences of expression of pnas-2 before and after treatment. It is concluded that the pnas-2 gene may be closely related with apotosis of arsenic sulfide treated APL cells, and may consider as a molecular biological remission marker in acute leukemia.
Full text:
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Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Arsenicals
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Sulfides
/
Tumor Cells, Cultured
/
Leukemia, Myeloid, Acute
/
Leukemia
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Antineoplastic Combined Chemotherapy Protocols
/
Apoptosis
/
K562 Cells
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article