Application of EGFP as reporter gene in study of WT1 regulation elements / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 599-602, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-276864
ABSTRACT
In order to investigate the feasibility of using EGFP as a reporter gene in WT1 transcriptional regulation study. WT1 promoter and enhancer were ligated into the vector pEGFP-1 by recombinant DNA technique and confirmed by restriction enzymes digestion. The resultant constructs were transfected into K562 cell line by DMRIE-C reagent and the function of these WT1 gene elements was detected by using a fluorescent microscope after transfection for 48 hours. The results indicated that the recombinant vectors, pEWP containing WT1 promoter, and pEWPE, pEWPA and pEWPD harboring both WT1 enhancer and promoter, had been successfully constructed. Fluorescence was observed in K562 cells transfected by pEWP, pEWPE, pEWPA and pEWPD, while no fluorescence could be detected in cells transfected by pEGFP-1. It is concluded that EGFP gene as a reporter gene can be applied to the WT1 transcriptional regulation study, which provides the basis for gene therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Enhancer Elements, Genetic
/
Genes, Reporter
/
K562 Cells
/
WT1 Proteins
/
Green Fluorescent Proteins
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article
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