Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 661-665, 2007.
Article
in English
| WPRIM
| ID: wpr-277348
ABSTRACT
The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences indicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Fungal Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Gene Expression
/
Transcriptional Activation
/
Promoter Regions, Genetic
/
Cloning, Molecular
/
Magnaporthe
/
Hyphae
/
Genetics
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2007
Type:
Article
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