Application of fluorescence in-situ hybridization and reverse transcription-polymerase chain reaction in molecular diagnosis of Ewing's sarcoma and primitive neuroectodermal tumor / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 328-332, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-277405
ABSTRACT
<p><b>OBJECTIVE</b>To detect tumor specific chromosome translocations and associated fusion transcripts in paraffin-embedded tissue by interphase fluorescence in-situ hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR), respectively, and to evaluate their diagnostic values for Ewing's sarcoma/primitive neuroectodermal tumor (ES/PNET).</p><p><b>METHODS</b>Nuclei of the tumor cells and total RNA were extracted from 10 cases of ES/PNET. Interphase FISH was utilized to analyze the EWS gene translocation with a dual color, break apart probe (Vysis company). RT-PCR was used to detect t (11; 22) (q24; q12) and t (21; 22) (q22; q12) fusion transcripts.</p><p><b>RESULTS</b>Among 10 cases of ES/PNET, the EWS-FLI1 fusion transcript was detected in 8 by RT-PCR. EWS-ERG fusion transcript was not detected in any of the cases. EWS gene translocation was found in 9 of 10 cases by FISH.</p><p><b>CONCLUSIONS</b>Interphase FISH and RT-PCR can be reliably applied to paraffin-embedded tissues for molecular diagnosis of ES/PNET. Between the two approaches, interphase FISH provides a more sensitive and stable result.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sarcoma, Ewing
/
Polymerase Chain Reaction
/
In Situ Hybridization, Fluorescence
/
Neuroectodermal Tumors, Primitive, Peripheral
/
Reverse Transcriptase Polymerase Chain Reaction
/
Diagnosis
/
Genetics
/
Methods
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Child
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2006
Type:
Article
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