Effect of epigallocatechin-3-gallate on the invasiveness of hepatocarcinoma cells in vitro / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>The effect of EGCG on the invasion of HepG2 cells in vitro was studied.</p><p><b>METHODS</b>The expressions of MUC1 in HepG2 cells before and after they were treated with EGCG were studied with immunohistochemistry. The effects of EGCG on the invasion of HepG2 cells were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membranes (Matrigel). Gelatin zymography was performed to detect the secretion of MMP-2 and MMP-9.</p><p><b>RESULTS</b>EGCG reduced the expression of MUC1, suppressed significantly the invasion of tumor cells into the basement membranes (P < 0.01) and reduced the secretion of MMP-2, MMP-9.</p><p><b>CONCLUSION</b>EGCG has anti-invasion effects on HepG2 cells.</p>