Ectopic Expression of Ephrin-A5 Under the EphA8 Promoter at the Anterior Region of the Superior Colliculus
Experimental Neurobiology
;
: 49-53, 2010.
Article
in English
| WPRIM
| ID: wpr-27764
ABSTRACT
EphA/ephrin-A mediated signaling has emerged as a key mechanism regulating axon guidance and topographic mapping, particularly in the well-characterized visual system from the retina to the superior colliculus (SC). In this study, EphA8 bacterial artificial chromosome (BAC) was manipulated to contain a floxed eGFP and human ephrin-A5 expression cassette using homologous recombination method. In the mice containing the recombinant BAC, it was shown that GFP is expressed in an anterior>posterior gradient in the SC. Furthermore, when these mice were crossed with the transgenic mice expressing Cre under the EphA8 promoter, it was evident that a GFP expression cassette was eliminated, and that human ephrin-A5 was ectopically expressed in the anterior region of the SC. This transgenic model would be useful to analyze the role of ephrin-A5 in the SC during the retinocollicular topography formation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retina
/
Axons
/
Mice, Transgenic
/
Superior Colliculi
/
Chromosomes, Artificial, Bacterial
/
Ephrin-A5
/
Homologous Recombination
Limits:
Animals
/
Humans
Language:
English
Journal:
Experimental Neurobiology
Year:
2010
Type:
Article
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