Molecular identification of heparin from pigs by allele-specific PCR (AS-PCR) and amplification refractory mutation system (ARMS) / 药学学报
Acta Pharmaceutica Sinica
;
(12): 535-541, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-277817
ABSTRACT
The aim of this study is to develop a convenient and effective method for the identification of heparin from pigs (include Sus scrofa domestica Brisson and Sus scrofa riukiuanus). Based on sequences of D-loop region of pigs and the other animals, two pairs of highly specific primers were designed for distinguishing heparin of pigs from other animals. The primers were employed to amplify D-loop region of DNA templates extracted from pig and seven other animal species that amounted to 49 samples. AS-PCR (allele-specific PCR) and ARMS (amplification refractory mutation system) were all suitable for fast identification of heparin from pig with anneal temperature at 54-56 degrees C in AS-PCR and with wider anneal temperature in ARMS,at 52-58 degrees C. An about 170 bp DNA fragments were amplified from separately pigs and whereas no DNA fragment was amplified from other samples under the same reaction condition.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Quality Control
/
Ruminants
/
DNA, Mitochondrial
/
DNA Mutational Analysis
/
Molecular Sequence Data
/
Base Sequence
/
Heparin
/
Polymerase Chain Reaction
/
Drug Contamination
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2008
Type:
Article
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