Pleiotrophin stimulates growth and albumin secretion of rat hepatocytes in culture / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 631-634, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-278026
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of pleiotrophin (PTN) on the growth of rat hepatocytes.</p><p><b>METHODS</b>Primary rat hepatocytes were isolated from male Sprague-Dawley rats and divided into three groups group A (negative control), cultivated in normal culture medium; group B (positive control), cultivated with culture medium supplemented with supernatant from the embryonic fibroblast 3T3 cell line; group C (experimental), cultivated with culture medium supplemented with human recombinant (hr) PTN (100 ng/ml). The hepatocytes' growth rate and level of secreted albumin (ALB) were evaluated by microscopy and biochemical assay, respectively. Significance of between-group differences were assessed by one-way ANOVA, and pairwise comparisons were performed by the least significant difference test.</p><p><b>RESULTS</b>The growth rates of hepatocytes in groups A, B and C were 2.800+/-0.084%, 4.300+/-0.132% and 3.800+/-0.053%, respectively. The growth rate of group B was significantly higher than the other two groups (F = 333.735, P less than 0.05). For all groups, the highest levels of secreted ALB were detected between the second and sixth day of culture, with g/L concentrations at day 2, 4 and 6 of group A, 0.550+/-0.010, 0.900+/-0.030 and 0.300+/-0.040; group B, 0.900+/-0.030, 1.300+/-0.020 and 1.400+/-0.030; group C, 0.900+/-0.010, 1.160+/-0.010 and 0.700+/-0.050. The secreted ALB of group B was significantly higher than that of the other two groups (F = 651.355, 338.831 and 863.205, P less than 0.05 ).</p><p><b>CONCLUSION</b>PTN can benefit in vitro culturing of rat hepatocytes by stimulating growth and enhancing their ability to secrete albumin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Carrier Proteins
/
Cells, Cultured
/
Cytokines
/
Rats, Sprague-Dawley
/
Bodily Secretions
/
Hepatocytes
/
Cell Biology
/
Albumins
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2013
Type:
Article
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