Gene therapy of rat prolactinomas mediated by adenoviral vectors with rat tyrosine hydroxylase gene / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 185-189, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-278100
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential of gene therapy of rat prolactinomas mediated by adenoviral vectors with a gene encoding rat tyrosine hydroxylase.</p><p><b>METHODS</b>Recombinant replication-deficient adenovirus named Ad-GFP-TH with rat TH-cDNA and control adenovirus named Ad-GFP were constructed by homologous recombination in bacterial cells. The rat pituitary prolactinoma cell line MMQ are chosen as the target cells to study the effect of gene therapy on their growth and prolactin secretion mediated by Ad-GFP-TH.</p><p><b>RESULTS</b>Recombinant Ad-GFP-TH and Ad-GFP were successfully reconstructed. Transfection of MMQ cells with Ad-GFP-TH not only restrained their growth but also decreased their PRL secretion.</p><p><b>CONCLUSION</b>Gene therapy may serve for a potential treatment for prolactinomas, especially invasive prolactinomas.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pituitary Neoplasms
/
Therapeutics
/
Tyrosine 3-Monooxygenase
/
Recombinant Proteins
/
Transfection
/
Genetic Therapy
/
Prolactinoma
/
Adenoviridae
/
Genetic Vectors
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2003
Type:
Article
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