Molecular cloning of Plasmodium yoelii dynamin-like protein (PyDyn) gene and the immunological character of its domains / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 176-180, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-278102
ABSTRACT
<p><b>OBJECTIVE</b>To identify and clone a new full ORF gene of PyDyn (Plasmodium yoelii dynamin-like protein), and examine the protection of their expression products.</p><p><b>METHOD</b>Using the P. yoelii Genome technology and RT-PCR.</p><p><b>RESULTS</b>The full ORF gene of PyDyn was amplified from mRNA of the erythrocytic stage of P. yoelii., three domains of PyDyn were expressed in E. coli., and the fairly positive immunogenicity of them was showed by IFA. The full ORF gene of PyDyn was 2,433 bp and encode 811 amino acids. Its Gene Bank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property.</p><p><b>CONCLUSION</b>The new full ORF gene of PyDyn is obtained and identified; their expressed domains are probably new candidates for malaria vaccine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Plasmodium yoelii
/
Vaccines, Synthetic
/
Base Sequence
/
Protozoan Proteins
/
Chemistry
/
Amino Acid Sequence
/
Cloning, Molecular
/
Genes, Protozoan
/
Malaria Vaccines
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2003
Type:
Article
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