Expression of rat protamine gene in MEL cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 601-605, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-278129
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of rat protamine (RP) gene in MEL cells and the effect on cell growth.</p><p><b>METHODS</b>Eukaryotic expression plasmid pCR-3.1-RP was constructed and transfected into MEL cells. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.</p><p><b>RESULTS</b>Transfected MEL cells showed lower growth rate, mitotic index and colony-forming rate. Volumes of cells were reduced and reduction of RNA transcription was observed.</p><p><b>CONCLUSION</b>These results suggest that expression of RP in MEL cell may inhibit the cell growth and proliferation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Plasmids
/
Tumor Cells, Cultured
/
Protamines
/
Transfection
/
Leukemia, Erythroblastic, Acute
/
Cell Division
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article
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