Establishment of drug-resistance cell line of human glioma mediated by MGMT / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 596-600, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-278130
ABSTRACT
<p><b>OBJECTIVE</b>To establish a drug-resistance cell line of human glioma mediated by MGMT.</p><p><b>METHODS</b>Simulated the clinical usage of BCNU to establish a BCNU-resistant human glioma subline by cyclic exposing the U251 parent cells to a constant concentration of BCNU. The resistance index and the expression of MGMT mRNA of U251/BCNU were detected and compared the difference of in vitro proliferation between U251 and U251/BCNU.</p><p><b>RESULTS</b>A subline--U251/BCNU was successfully established in about 4-month culture, which had a stable resistance to BCNU. U251/BCNU cells showed 17-fold higher resistance to BCNU than did U251 cells by MTT assay, while U251/BCNU cells expressed MGMT mRNA. The doubling time of U251 and U251/BCNU had no statistical difference.</p><p><b>CONCLUSION</b>A drug-resistance cell line of human glioma mediated by MGMT is established, which could provide experimental basis for further studies on the resistance mechanism and reversal methods of glioma chemotherapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Brain Neoplasms
/
DNA Modification Methylases
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Neoplasm
/
O(6)-Methylguanine-DNA Methyltransferase
/
Cell Line, Tumor
/
Genetics
/
Glioma
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article
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