Architecture, influencing factors, and sensitivity to antifungal agents of Candida biofilm / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 385-388, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-278158
ABSTRACT
<p><b>OBJECTIVE</b>To establish a model of Candida biofilm and to explore its characteristics, ultrastructure, influences by saliva and serum, and sensitivity to antifungal agents.</p><p><b>METHODS</b>Evaluations of the in vitro growth kinetics, influences by saliva and serum, and sensitivity to antifungal agents of Candida biofilm were performed with the abated tetrazolium salt XTT method on a 96-well microtire petri dish. The ultrastructure of Candida biofilm was observed under Confocal Laser Scanning Microscope (CLSM).</p><p><b>RESULTS</b>The bioactivity of Candida biofilm increased with culturing time and serum could obviously increase the action of biofilm. The Candida biofilm was significantly resistant to routine antifungal agents.</p><p><b>CONCLUSION</b>The Candida cells adhered in biofilms are significantly different in morphology from those in suspension and are resistant to routine antifungal agents such as Amphotericine B, Fluconazole and Itraconazole.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Candida
/
Microbial Sensitivity Tests
/
Fluconazole
/
Amphotericin B
/
Itraconazole
/
Microscopy, Confocal
/
Biofilms
/
Drug Resistance, Fungal
/
Antifungal Agents
Type of study:
Prognostic study
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article
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