An observation of taurine transport alterations in calcification of myocardial cells in vitro / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 359-363, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-278165
ABSTRACT
<p><b>OBJECTIVE</b>To observe the alterations of taurine transport, taurine transporter (TAUT) and cysteine sulfinate decarboxylase (CSD) mRNA in the calcification of myocardial cells in vitro.</p><p><b>METHODS</b>3H-taurine measured the amount of taurine uptake. TAUT and CSD mRNA consents were measured using competitive quantitative RT-PCR in cultured and calcified myocardial cells.</p><p><b>RESULTS</b>In calcification of myocardial cells, taurine concentration was decreased by 27% (P < 0.05), taurine uptake was markedly reduced, Vmax reduced by 39% (P < 0.01), there were no statistical significance of Km values between the two groups. TAUT mRNA decreased by 45% (P < 0.01), but CSD mRNA increased by 25% (P < 0.05).</p><p><b>CONCLUSIONS</b>The data suggest that there were impediment of taurine transport in calcification of myocardial cells, as TAUT mRNA level was decreased, but CSD mRNA concentration was improved.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Taurine
/
Biological Transport
/
Calcinosis
/
RNA, Messenger
/
Carboxy-Lyases
/
Cells, Cultured
/
Calcium
/
Myocytes, Cardiac
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article
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