Cloning cDNA encoding for GAGA-like element binding proteins in human Jurkat cells / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore GAGA-like element binding protein in human cells.</p><p><b>METHODS</b>Yeast one-hybrid system was used to screen the GAGA-like element binding proteins in HTLV-1 transformed Jurkat cell cDNA fusion library. Total RNA extracted from Jurkat cells was first labeled by reverse transcription, and was taken as cDNA probe to hybridize with the candidate positive clones.</p><p><b>RESULTS</b>9 positive clones were obtained, and 6 out of the 9 clones were positively hybridized with the cDNA probe.</p><p><b>CONCLUSIONS</b>6 candidate clones encoding for GAGA-like element binding proteins were obtained from Jurkat cells for further investigation.</p>