Identification of a novel HLA-A allele A*11:01:37 by sequence-based typing / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify a novel HLA-A allele in a Chinese Han individual.</p><p><b>METHODS</b>One mismatch was observed in HLA-A locus in HLA typing for CMDP donors using bi-allelic SBT kit. A confirmatory test for novel HLA allele was performed with mono-allelic SBT kit.</p><p><b>RESULTS</b>The DNA sequence was confirmed to be a novel HLA-A allele. There was 1 nucleotide differed from the closest matching HLA-A*110101 at position 393(G→A), which resulting a change from GGG to GGA at codon 107, led to a silent mutation, conserving the amino acid Gly.</p><p><b>CONCLUSION</b>A novel HLA-A allele was confirmed and officially named HLA-A*110137 (Genbank accession number, JN209962) by the WHO Nomenclature Committee for Factors of the HLA System in January 2012.</p>