Quantitative PCR for early diagnosis of invasive fungal infections in patients with hematologic malignancies / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1225-1230, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-278401
ABSTRACT
This study was aimed to establish the approach of quantitative PCR (q-PCR) for diagnosis of invasive fungal infections (IFI) in patients with hematologic malignancies. Specimens from 40 patients with hematologic malignancies were chosen for q-PCR and galactomannan (GM) test. The 28S rRNA, a real high consensus sequence of fungi, was selected as target gene to design primer and probe. The DNA of fungal species was extracted from serum specimens. The results showed that q-PCR sensitivity, specificity, positive and negative predictive values were 0.89, 0.85, 0.89, 0.85 respectively; GM test sensitivity, specificity, positive and negative predictive values were 0.83, 0.80, 0.88, 0.73 respectively; as combined q-PCR with GM test, these values were 0.94, 0.85, 0.89, 0.92 respectively. It is concluded that the q-PCR assay can be used for early diagnosis for IFI in patients with hematologic malignancies, q-PCR combined with GM test can enhance the diagnosis sensitivity for IFI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymerase Chain Reaction
/
Predictive Value of Tests
/
Sensitivity and Specificity
/
Hematologic Neoplasms
/
Early Diagnosis
/
Diagnosis
/
Fungi
/
Methods
/
Microbiology
/
Mycoses
Type of study:
Diagnostic study
/
Screening study
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2012
Type:
Article
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