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Diagnostic value of fluorescence in situ hybridization for children with acute myeloid leukemia / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1099-1102, 2012.
Article in Chinese | WPRIM | ID: wpr-278427
ABSTRACT
This study was purposed to investigate the diagnostic value of fluorescence in situ hybridization (FISH) technique for the childhood acute myeloid leukemia (AML). The medical data of 179 children with AML (aged ≤ 16 years) were retrospectively studied, who were initially diagnosed in our hospital from April 2005 to April 2010. Through the analysis of the results of FISH, chromosome banding analysis and polymerase chain reaction, the difference and complementarity between FISH and other 2 methods for detecting the fusion genes were explored. The results indicated that the detection rate of genetic abnormality with FISH was higher. The PML/RARα probe was used in 27 AML-M3 patients, 22 out of whom were evaluated as PML/RARα positive. The AML1/ETO probe was used in 24 AML-M2b patients and all of them were evaluated as AML1/ETO positive. The CBFβ/MYH11 probe was used in 4 AML-M4Eo patients and all of them were evaluated as CBFβ/MYH11 positive. It is concluded that FISH is a sensitive method for detecting fusion genes and the results of FISH have a good correlation with the chromosome banding analysis and polymerase chain reaction. The combination of FISH with other 2 methods improves the detection rate of genetic abnormality, which is useful for the diagnosis and typing of childhood AML.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Leukemia, Myeloid, Acute / Retrospective Studies / In Situ Hybridization, Fluorescence / Diagnosis / Genetics / Karyotyping Type of study: Diagnostic study / Observational study Limits: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male Language: Chinese Journal: Journal of Experimental Hematology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Leukemia, Myeloid, Acute / Retrospective Studies / In Situ Hybridization, Fluorescence / Diagnosis / Genetics / Karyotyping Type of study: Diagnostic study / Observational study Limits: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male Language: Chinese Journal: Journal of Experimental Hematology Year: 2012 Type: Article