Establishment of a new method for screening of CBFB-MYH11 fusion gene in acute myeloid leukemia and its value in clinical use / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1077-1081, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-278432
ABSTRACT
This study was purposed to establish new method for detecting CBFB-MYH11 fusion gene in acute myeloid leukemia (AML) and to evaluate its value in clinical use. All fusion types of reported CBFB-MYH11 fusion gene were defined by search of references and databank, then the primers and probes were designed on this basis, and 3 positive plasmids and negative cell line as control were established. GUSB gene was also amplified as an internal reference. The primer/probe sets were tested with 3 positive plasmids and HL-60 cDNA using quantitative real-time PCR (qPCR) assays, which were then combined as a multiplex qPCR for simultaneous detection of CBFB-MYH11 and GUSB. After optimization, the multiplex qPCR assay demonstrated both high sensitivity (10 copies for all the 3 plasmids) and high specificity. Finally, the multiplex qPCR assay was clinically evaluated with 58 AML patients, and 4 CBFB-MYH11-positive cases (6.9%) were detected, involving A type (3 cases) and J type (1 case). By comparison, the multiplex qPCR assay showed results concordant with sequencing results, and detected one case that was missed by cytogenetic analysis. It is concluded that a novel qPCR method for screening of CBFB-MYH11 fusion gene in AML is established. This method is fast, comprehensive, sensitive, specific, reliable, and should consider to be a robust tool for identification and management of AML patients with CBFB-MYH11 fusion gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukemia, Myeloid, Acute
/
Case-Control Studies
/
Oncogene Proteins, Fusion
/
Myosin Heavy Chains
/
HL-60 Cells
/
Diagnosis
/
Core Binding Factor beta Subunit
/
Real-Time Polymerase Chain Reaction
/
Genetics
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2012
Type:
Article
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