Identification of a novel HLA allele, HLA-B*35:03:07, by sequencing-based typing / 中国实验血液学杂志

ABSTRACT

This study was purposed to analyze and identify a novel HLA allele in Chinese population. A new HLA-B allele which is closely related to HLA-B*350301 was initially detected by PCR-SSOP, then DNA sequencing was performed to identify the difference between the novel allele and HLA-B*350301 allele. The result showed that the sequence of the new allele was different from all other known sequence. It differs from the closest matching HLA-B*350301 by a single substitution at position 387 C→G in exon 3, no resulting in amino acid change. It is concluded that this allele is a novel one and has been officially named B*350307 by the WHO Nomenclature Committee.