Effect of sphingosine 1-phosphate/sphingosine 1-phosphate receptor signal pathway on function of neutrophils / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 989-994, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-278450
ABSTRACT
The aim of this study was to examine the priming effect of sphingosine 1-phosphate (S1P) on fMLP-activated neutrophils, mainly to detect the neutrophil respiratory burst products, and to investigate the signaling pathway involved in S1P activity. Flow cytometry was used to evaluate the new isolated neutrophil; the superoxide anion output was detected indirectly by cytochrome C reduction in respiratory burst; the dihydro-rhodamine 123 was used to detect the intensity of respiratory burst; the signal transduction pathways of neutrophil respiratory burst were explored by Western blot. The results showed that after pretreated with S1P, the level of superoxide anion released by fMLP-activated neutrophils significantly increased; the Rhodamine 123 mean fluorescence intensity in S1P primed fMLP-activated neutrophils group was significantly higher than that in fMLP treatment group; PI3K and Akt proteins involved in the signal pathway of neutrophil respiratory burst. It is concluded that S1P is a new priming reagent, which primes respiratory burst of fMLP-activated neutrophils; this signal pathway may be that S1P interacts with its receptor, activates PI3K, then activates Akt-transmitting signals through NADPH oxidase, finally results in the respiratory burst.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Sphingosine
/
Lysophospholipids
/
Signal Transduction
/
Cells, Cultured
/
Respiratory Burst
/
Superoxides
/
NADPH Oxidases
/
Receptors, Lysosphingolipid
/
Proto-Oncogene Proteins c-akt
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2012
Type:
Article
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