AG490 inhibits the proliferation of K562 and down-regulates protein phosphatase PHLPP expression / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 889-892, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-278471
ABSTRACT
This study was aimed to investigate the effect of AG490, a JAK2 inhibitor, on expression of PHLPP and p-Akt in K562. K562 cells were treated with different concentrations of AG490. The proliferation of K562 cells was examined by WST-1 assay and apoptosis of K562 cells was detected by flow cytometry with Annexin V-FITC/PI double staining. The expressions of PHLPP, phosphorate-Akt (p-Akt) and total Akt protein were detected by Western blot. The results indicated that AG490 inhibited the proliferation of K562 cells in concentration-and time-dependent manners, with the IC(50) 338.0 µmol/L in 48 h. AG490 100 µmol/L also induced apoptosis of K562 cells in a time-dependent manner. AG490 100 µmol/L time-dependently down-regulated the protein expression of p-Akt and PHLPP, but without significant effect on expression of total Akt. It is concluded that AG490 can inhibit proliferation and induce apoptosis of K562 cells through down-regulation of p-Akt expression, but inhibiting efficacy of AG490 on K562 proliferation also may be limited due to the down-regulation of p-Akt regulatory protein PHLPP expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Nuclear Proteins
/
Down-Regulation
/
Phosphoprotein Phosphatases
/
K562 Cells
/
Tyrphostins
/
Cell Proliferation
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2012
Type:
Article
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