Effects of leukemia bone marrow stromal cells on resistance of co-cultured HL-60 to idarubicin / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 163-165, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-278775
ABSTRACT
To study the role of hematopoietic microenvironment abnormality in development of minimal residual disease and its mechanism, the viability of HL-60 cells was investigated by means of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cell with HL-60 cells and idarubicin (IDA), flow cytometry and ELISA. The results showed that viability of HL-60 cells gradually decreased along with the increase of IDA dose and prolongation of culture time. Amount of HL-60 cells co-cultured with leukemia bone marrow stramal cells was significantly increased as compared with that of the control (P < 0.05). Bone marrow stromal cells or stromal cell conditioned medium reduced the effect of IDA on HL-60 cells in culture. In conclusion, leukemia bone marrow stromal cells contribute to increasing resistance of HL-60 cells to chemotherapeutic agents, and play some role in developing minimal residual disease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Bone Marrow Cells
/
Idarubicin
/
Cell Survival
/
Stromal Cells
/
HL-60 Cells
/
Drug Resistance, Neoplasm
/
Coculture Techniques
/
Dose-Response Relationship, Drug
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2004
Type:
Article
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