The effects of interferon-gamma on the expression of the cyclin D isoforms in cord blood hematopoietic stem/progenitor cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 138-141, 2004.
Article
in English
| WPRIM
| ID: wpr-278781
ABSTRACT
To explore the hematopoiesis inhibition mechanisms of interferon-gamma (IFN-gamma), the effects of IFN-gamma on the expression of the cyclin D in the umbilical cord blood hematopoietic stem/progenitor cells were observed. In the experiments the CD34(+) cells were isolated from the cord blood with MIDI-MACS system; semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; the expression levels of cyclin D isoforms were assayed by semi-quantitative RT-PCR, after the hematopoietic stem/progenitor cells were incubated with IFN-gamma. The results indicated that IFN-gamma could inhibit the formation of CFU-GM and down-regulate the expression of cyclin D2 and cyclin D3 at the mRNA level. It is concluded that the IFN-gamma could inhibit the proliferation of hematopoietic stem cells and down-regulate the expression of cyclin D, that may be one mechanism underlying the hematopoietic inhibition of IFN-gamma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Hematopoietic Stem Cells
/
G1 Phase
/
Interferon-gamma
/
Cyclins
/
Protein Isoforms
/
Cell Biology
/
Cyclin D
/
Fetal Blood
Limits:
Humans
Language:
English
Journal:
Journal of Experimental Hematology
Year:
2004
Type:
Article
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