The reverse effect on drug-resistance against tyrosine kinase inhibitor STI571 in mdr1 and bcr-abl positive leukemic cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 600-603, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-278830
ABSTRACT
To explore the possibility of leukemia cell line of both bcr-abl and mdr-1 positive were cross-resistant to tyrosine kinase inhibitor STI571 and its reversal way, the inhibitory effect of STI571 on K562-n/VCR cells was detected with MTT method and reverse effects of CsA, TAM, IFN-alpha and CsA cominated with IFN-alpha were observed. The results showed that K562-n/VCR cell line expressing bcr-abl and mdr1 positive was resistant to STI571, and could be reversed by 5.18, 1.82 and 1.67-fold respectively, when treated with CsA, TAM, and IFN-alpha. It could be reversed by 34.87-fold with combination of half-dose CsA and IFN-alpha. In conclusion, amplification of mdr1 gene may contribute to drug-resistance of bcr-abl positive leukemic cells against STI571. The reversal agents, CsA, TAM and IFN-alpha show obviously reverse effects on drug-resistance. The combination of half-dose of both CsA and IFN-alpha display stronger effect than the full dose of either.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Piperazines
/
Pyrimidines
/
Tamoxifen
/
Benzamides
/
Leukemia
/
Genes, abl
/
Interferon-alpha
/
Cyclosporine
/
Drug Resistance, Neoplasm
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2003
Type:
Article
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