Human placenta derived adherent cells support in vitro expansion of umbilical cord blood CD34+ cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 560-564, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-278839
ABSTRACT
The objective of this study was to elucidate the effect of human placenta adherent cells (hPDAC) on expansion of human umbilical cord blood CD34(+) cells in vitro. hPDAC was isolated and characterized in human placenta tissue by using enzyme-digesting method and flow cytometry. A co-culture system was established with hPDAC and cord blood CD34(+) cells. The CD34(+) cells were cultured in different culture systems with different combinations of hPDAC and SCF, IL-3, IL-6 and FL. The number of total nucleated cells, CFC and CD34(+) cells were repeatedly counted in culture for 4 weeks. The results showed that the hPDAC displayed fibroblast-like morphology, and were positive for CD29, CD44, CD166, HLA-ABC and UEA-1, and negative for CD34, CD45 and HLA-DR. Functionally, ex vivo expansion of CD34(+) cells on feeder layer of placental adherent cells was significantly higher than that no feeder layer group. SCF + IL-3 + IL-6 + FL + hPDAC manifested the most potent combination, with the number of total nucleated cells increasing by (126.0 +/- 6.7)-fold, progenitor cells (CFC) (49.8 +/- 1.7)-fold and CD34(+) cells (8.3 +/- 1.65)-fold. It is concluded that placental adherent cells could support hematopoiesis in vitro and work as a suitable feeder layer for cord blood stem/progenitor cell expansion.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Hematopoietic Stem Cells
/
Cell Adhesion
/
Cell Separation
/
Antigens, CD34
/
Cell Biology
/
Fetal Blood
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2003
Type:
Article
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