Real-time quantitative PCR detecting minimal residual disease in multiple myeloma patients after autologous peripheral blood stem cell transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 516-520, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-278850
ABSTRACT
In order to explore the role of real-time PCR in detecting minimal residual disease in multiple myeloma and Waldenstrom's macroglobulinemia after autologous peripheral blood stem cell transplantation (APBSCT), real-time PCR was used to quantitate the IgH rearrangement in 8 patients with multiple myeloma (MM) and 1 case of Waldenstrom's macroglobulinemia before and after APBSCT. The results showed that the copies of IgH rearrangement pre- or post-APBSCT were 3108 +/- 1043 and 549 +/- 660 (P < 0.05) respectively. The number of IgH copies was positively correlated with the amount of plasmocytes in patient 's bone marrow and the M-protein in peripheral blood (r = 0.86, P < 0.05). Similar result was obtained in a case of relapsed Waldenstrom's macroglobulinemia. In conclusion, the quantitative analysis of IgH rearrangement by real-time PCR is a novel way to evaluate the therapeutic efficaciousness and predict the prognoses in MM patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Transplantation, Autologous
/
Gene Rearrangement
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Immunoglobulin Heavy Chains
/
Neoplasm, Residual
/
Peripheral Blood Stem Cell Transplantation
/
Diagnosis
/
Genetics
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2003
Type:
Article
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