Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 160-161, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-278989
ABSTRACT
<p><b>BACKGROUND</b>To search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.</p><p><b>METHODS</b>Quantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay.</p><p><b>RESULTS</b>Taqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower.</p><p><b>CONCLUSIONS</b>Sybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organic Chemicals
/
Blood
/
DNA, Viral
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Fluorescent Dyes
/
Genetics
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2002
Type:
Article
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