Roles of follicular helper T cells and follicular regulatory T cells in pathogenesis of Henoch-Schönlein purpura in children / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 1084-1087, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-278993
ABSTRACT
<p><b>OBJECTIVE</b>To study the roles of follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells in the pathogenesis of Henoch-Schönlein purpura (HSP) in children.</p><p><b>METHODS</b>Peripheral blood samples were collected from 40 HSP children and 25 healthy controls. The percentages of Tfh and Tfr cells were measured by flow cytometry; the mRNA expression levels of Bcl-6, c-MAF, Blimp-1, and PD-1 in peripheral blood were measured by real-time polymerase chain reaction.</p><p><b>RESULTS</b>Compared with the controls, the children with HSP had significantly increased percentage of Tfh cells and Tfh/Tfr ratio but a significantly reduced percentage of Tfr cells in the peripheral blood (P<0.05). Compared with the controls, the children with HSP had significantly increased mRNA expression of Bcl-6 and c-MAF but significantly reduced mRNA expression of Blimp-1 in CD4+ T cells (P<0.05), and had significantly increased mRNA expression of PD-1 but significantly reduced mRNA expression of Blimp-1 in CD4+CD25+ regulatory T cells (P<0.05).</p><p><b>CONCLUSIONS</b>Abnormal percentages of Tfh and Tfr cells may be involved in the pathogenesis of HSP in children, and over-expression of Bcl-6, c-MAF, and PD-1 mRNA and inhibited expression of Blimp-1 mRNA may be considered as important reasons for abnormal percentages of Tfh and Tfr cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
IgA Vasculitis
/
T-Lymphocytes, Regulatory
/
T-Lymphocytes, Helper-Inducer
/
DNA-Binding Proteins
/
Allergy and Immunology
/
Proto-Oncogene Proteins c-maf
/
Proto-Oncogene Proteins c-bcl-6
/
Programmed Cell Death 1 Receptor
/
Genetics
Type of study:
Etiology study
Limits:
Adolescent
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS