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Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 591-594, 2005.
Article in Chinese | WPRIM | ID: wpr-279105
ABSTRACT
<p><b>OBJECTIVE</b>To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae.</p><p><b>METHOD</b>The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.521.576) and detection wavelength was 347 nm.</p><p><b>RESULT</b>The arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good (r = 0.9992, 0.9999, 0.9997, 0.9997) in ranges of 0.035-0.56, 0.043-0.69, 0.040-0.64 and 0.040-0.64 microg, respectively. The average recoveries were 96.8%, 97.3%, 96.8%, 98.5% and RSD the values of repeatability were 2.5%, 3.0%, 1.5%, 3.2%, respectively.</p><p><b>CONCLUSION</b>The method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Chemistry / Chromatography, High Pressure Liquid / Artemisia / Flowers / Flavones Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Chemistry / Chromatography, High Pressure Liquid / Artemisia / Flowers / Flavones Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2005 Type: Article