Quantitative analysis of the nucleosides in Cordyceps sinensis with capillary zone electrophoresis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 447-449, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-279143
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quantitative analysis method for analyzing the nucleosides in Cordyceps sinensis with capillary electrophoresis, and compare the difference between natural and the cultured C. mycelia.</p><p><b>METHOD</b>Capillary zone electrophoresis method was employed to quantitate the adenosine, uridine, guanosine and inosine in C. sinensis, with 0.25 mg x L(-1) boric acid-sodium hydroxide buffer, pH 9.5. The working voltage was 20 kV, the temperature was 25 degrees C, and the detection wavelength was 260 nm.</p><p><b>RESULT</b>With the capillary zone electrophoresis method, the average recovery of the above 4 nucleosides was 98.9%, 95.1%, 97.8% and 98.8% respectively, with the RSD 0.4%, 1.7%, 1.3% and 5.0%. There was no adenosine in natural C. sinensis and no inosine in the cultured C. mycelia detected.</p><p><b>CONCLUSION</b>This method can be used to determine the adenosine, uridine, guanosine and inosine in C. sinensis. The nucleosides in C. sinensis produced from Qinghai province and cultured C. mycelia are obviously different.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Uridine
/
Adenosine
/
Chemistry
/
Classification
/
Electrophoresis, Capillary
/
Cordyceps
/
Culture Techniques
/
Guanosine
/
Inosine
/
Lepidoptera
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2005
Type:
Article
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