Effects of Epimedium pubescens icariine on proliferation and differentiation of human osteoblasts / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 289-291, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-279175
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of epimedium pubescens icariine on the proliferation and differentiation of human osteoblasts.</p><p><b>METHOD</b>Human osteoblasts were obtained by inducting human marrow mesenchymal stem cells (hMSCs) directionally. MTT was used to observe the proliferation and activity of ALP was assayed to observe the differentiation of the third passage human osteoblasts cultured in vitro. The expression of BMP-2 mRNA was checked by RT-PCR.</p><p><b>RESULT</b>Epimedium pubescens icariine at the dose of 20 microg x mL(-1) increased greatly the proliferation and differentiation of human osteoblasts and promoted the expression of BMP-2 mRNA.</p><p><b>CONCLUSION</b>Epimedium pubescens icariine enhances significantly the proliferation and differentiation of human osteoblasts, which may be mediated by increasing the expression of BMP-2 mRNA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pharmacology
/
Plants, Medicinal
/
Flavonoids
/
RNA, Messenger
/
Cell Differentiation
/
Chemistry
/
Transforming Growth Factor beta
/
Bone Morphogenetic Proteins
/
Cell Biology
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2005
Type:
Article
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