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Research on effect of Sargentodoxae caulis on activity of osteoclasts and proliferation differentiation of osteoblasts / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4463-4468, 2015.
Article in Chinese | WPRIM | ID: wpr-279215
ABSTRACT
Through morphological observation, HE staining, TRAP staining and toluidine blue staining of bone resorption pits to identify osteoclasts which obtained by 1α, 25-(OH)2 VitD3 inducing rabbit bone marrow cells. Three indicators-TRAP staining, TRAP enzyme activity detecting and the number and area of bone resorption pits were adapted to detect the effect of Sargentodoxae caulis on the activity of osteoclasts. Culturing MC3T3-E1 Subclong 14 cells and detecting the effect of S. caulis on differentiation and proliferation of them by MTT and detecting the alkaline phosphatase in cells. The results show that all of the low, middle and high doses of water and alcohol extracts of S. caulis have significant inhibition on osteoclast differentiation and bone resorption ability in a dose-dependent manner. The low and middle doses of water and alcohol extracts of S. caulis can stimulate differentiation and proliferation of MC3T3-ElSubclone 14 cells, which indicates S. caulis can prevent osteoporosis and the function could be achieved by inhibiting osteoclast activity and promoting the proliferation and differentiation of osteoblasts.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Pharmacology / Bone Resorption / Drugs, Chinese Herbal / Cell Differentiation / Cells, Cultured / Cell Biology / Cell Proliferation / Drug Therapy Limits: Animals / Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Pharmacology / Bone Resorption / Drugs, Chinese Herbal / Cell Differentiation / Cells, Cultured / Cell Biology / Cell Proliferation / Drug Therapy Limits: Animals / Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article