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Multi-component quantitative analysis combined with chromatographic fingerprint for quality assessment of Onosma hookeri / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4442-4445, 2015.
Article in Chinese | WPRIM | ID: wpr-279219
ABSTRACT
A method for simultaneous determination of the shikonin, acetyl shikonin and β, β'-dimethylpropene shikonin in Onosma hookeri and the chromatographic fingerprint was estabished by HPLC-DAD on an Agilent Zorbax SB-column with a gradient elution of acetonitrile and water at 0.8 mL x min(-1), 30 degrees C. The quality assessment was conducted by comparing the content difference of three naphthoquinone constituents, in combination with chromatographic fingerprint analysis and systems cluster analysis among 7 batches of radix O. hookeri. The content of the three naphthoquinone constituents showed wide variations in 7 bathces. The similarity value of the fingerprints of sample 5, 6 and 7 was above 0.99, sample 2 and 3 above 0.97, sample 3 and 4 above 0.90, and other samples larger than 0.8, which was in concert with the content of three naphthoquinone constituents. The 7 samples were roughly divided into 4 categories. The results above indicated that the using of this medicine is complex and rather spotty. The established HPLC fingerprints and the quantitative analysis method can be used efficiently for quality assessment of O. hookeri.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Naphthoquinones / Plant Roots / Boraginaceae / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Naphthoquinones / Plant Roots / Boraginaceae / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article