Your browser doesn't support javascript.
loading
HPLC specific chromatogram spectrum-effect relationship for Shuanghuanglian on MDCK cell injury induced by influenza A virus (H1N1) / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4194-4199, 2015.
Article in Chinese | WPRIM | ID: wpr-279263
ABSTRACT
To establish HPLC specific chromatogram and its correlation with the protection effect of Shuanghuanglian on MDCK (Madin-Darby canine kidney) cell injury induced by influenza A virus( H1N1). Nine recipes of Shuanghuanglian based on the official prescription were prepared according to orthogonal test for HPLC analysis and MDCK cells protection experiment separately (cytopathic effect (CPE) method was used for observing the virus infectivity and MTT staining results were used as the determining indexes for drug concentration selection and analyzing cell viability). The results suggested that all the other Shuang-Huang-Lian recipes except recipe1 demonstrate protecting effect on MDCK cell injury induced by influenza A virus (P < 0.01, P < 0.001). Stepwise regression analysis was used for analyzing the relationships between HPLC fingerprint and the protecting effect of Shuanghuanglian on influenza A virus induced MDCK cell injury. Peak 2, 3, 6, 8 and 12 were found to be strongly related with anti-influenza A virus efficacy. Stepwise regression analysis of recipes data and efficacy data showed that Lonicerae Japonicae Flos and Forsythiae Fructus were positively associated with the protecting effect of cells injury. From HPLC fingerprints, we found that peak 2, 3, 12 were from Lonicerae Japonicae Flos and peak 6, 8 were from Forsythiae Fructus. Four peaks were identified through comparing the retention time between the standard and Shuanghuanglian recipes, and they were chlorogenicacid, cryptochlorogenic acid, forsythoside B and 3,4-dicaffeoylquinic acid respectively. Caffeic acid derivatives in Lonicerae Japonicae Flos and Forsythiae Fructus were found to be greatly correlated with anti-influenza A virus efficacy and maybe the substance basis of Shuanghuanglian.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Antiviral Agents / Pharmacology / Physiology / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Lonicera / Scutellaria baicalensis / Forsythia / Influenza A Virus, H1N1 Subtype Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Antiviral Agents / Pharmacology / Physiology / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Lonicera / Scutellaria baicalensis / Forsythia / Influenza A Virus, H1N1 Subtype Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article