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Cloning and expression analysis of 4- (cytidine-5-diphospho) -2-C-methyl-D-erythritol kinase gene in Tripterygium wilfordii / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4165-4170, 2015.
Article in Chinese | WPRIM | ID: wpr-279268
ABSTRACT
4-(Cytidine-5-diphospho) -2-C-methyl-D-erythritol kinase is a key enzyme in the biosynthesis pathway of terpenoids. According to the transcriptome database, the specific primers were designed and used in PCR. The bioinformatic analysis of the sequenced TwCMK gene was performed in several bioinformatics software. The Real-time fluorescence quantification polymerase chain reaction (RT-qPCR) were used to detect the expression levels of TwCMK from T. wilfordii after elicitor MeJA supplied. The results showed that the full length of TwCMK cDNA was 1 732 bp encoding 387 amino acids. The theoretical isoelectric point of the putative TwCMK protein was 5.79 and the molecular weight was about 42.85 kDa. MeJA stimulated the rising of TwCMK expression in suspension cell and signally impacted at 24 h. The research provides a basis for further study on the regulation of terpenoid secondary metabolism and biological synthesis.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Plant Proteins / Molecular Sequence Data / Models, Molecular / Chemistry / Sequence Alignment / Amino Acid Sequence / Cloning, Molecular / Phosphotransferases (Alcohol Group Acceptor) / Gene Expression Regulation, Plant Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Plant Proteins / Molecular Sequence Data / Models, Molecular / Chemistry / Sequence Alignment / Amino Acid Sequence / Cloning, Molecular / Phosphotransferases (Alcohol Group Acceptor) / Gene Expression Regulation, Plant Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article