Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells / 生理学报
Acta Physiologica Sinica
; (6): 103-106, 2002.
Article
in Zh
| WPRIM
| ID: wpr-279331
Responsible library:
WPRO
ABSTRACT
To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r=0.9703, P<0.01). (2) VIP (0.001-1 micromol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P<0.01). (3) Heat stress enhanced the secretion of VIP (P<0.01) and upregulated the expression of VIPR on BECs (P<0.05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Bronchi
/
Vasoactive Intestinal Peptide
/
Cells, Cultured
/
Chemotaxis
/
Receptors, Vasoactive Intestinal Peptide
/
Cell Biology
/
Epithelial Cells
/
Insulin
Limits:
Animals
Language:
Zh
Journal:
Acta Physiologica Sinica
Year:
2002
Type:
Article