DNA barcoding in medicinal plants Caprifoliaceae / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2527-2532, 2010.
Article
in Zh
| WPRIM
| ID: wpr-279407
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the candidate sequences which can be used as DNA barcode to identify species in Caprifoliaceae family by screening out from four different DNA fragments sequences.</p><p><b>METHOD</b>PCR amplification, sequencing efficiency, differential intra- and interspecific divergences, the DNA barcoding gap and identification efficiency were used to evaluate these loci.</p><p><b>RESULT</b>The ITS2 was used as a candidate sequence of DNA barcode to identify the species in Caprifoliaceae family, whose rate of success in identification in genera level was 100% and in species 96.6%, and psbA-trnH as a complementary barcode to ITS2 for Caprifoliaceae.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
/
Species Specificity
/
DNA
/
Electronic Data Processing
/
Base Sequence
/
Polymerase Chain Reaction
/
DNA, Plant
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Caprifoliaceae
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Genetics
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Identification, Psychological
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article