Purification of superoxide dismutase from blood erythrocyte by nano magnetic chitosan microspheres / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 990-994, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-279453
ABSTRACT
Nano magnetic microspheres prepared by chitosan and poly acylic acid were applied to purifying superoxide dismutase from blood erythrocyte. Chitosan-polyacyilc acid graft copolymer was synthesized by free radical graft copolymerization with potassium persulfate as inititator. To prepare Fe3O4 magnetic fluids with chemical coprecipitation, chitosan-polyacylic nano magnetic microspheres were prepared with glutaraldehyde as crosslinking agent. Structure of nano magnetic microspheres was detected by FT-IR spectrometer. Particle size and morphology were characterized by JEM-4000EX technology. Chitosan-polyacylic nanometer microspheres have good paticle cize distribution, magnetic responsiveness and protein adsoption. Activity, product yield and activity recovery of SOD after purification reached 6 727 U/mg, 21.1%, and 85.7% respectively. Purification of blood superoxide dismutase by chistosan-polyacylic acid microspheres has its renewable and feasible nature.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymers
/
Superoxide Dismutase
/
Chemistry
/
Glutaral
/
Spectroscopy, Fourier Transform Infrared
/
Chitosan
/
Erythrocytes
/
Magnetics
/
Microspheres
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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