Production of L(+)-tartaric acid by immobilized Rhizobium strain BK-20 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 315-319, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-279518
ABSTRACT
The cis-epoxysuccinate hydrolase (CESH) from Rhizobium strain BK-20 is the key enzyme for L(+)-tartaric acid production. To establish a highly efficient and stable production process, we first optimized the enzyme production from Rhizobium strain BK-20, and then developed an immobilized cell-culture process for sustained production of L(+)-tartaric acid. The enzyme activity of free cells reached (3 498.0 +/- 142.6) U/g, and increased by 643% after optimization. The enzyme activity of immobilized cells reached (2 817.2 +/- 226.7) U/g, under the optimal condition with sodium alginate as carrier, cell concentration at 10% (W/V) and gel concentration at 1.5% (W/V). The immobilized cells preserved high enzyme activity and normal structure after 10 repeated batches. The conversion rate of the substrate was more than 98%, indicating its excellent production stability.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rhizobium
/
Tartrates
/
Chemistry
/
Cells, Immobilized
/
Glucuronic Acid
/
Alginates
/
Hexuronic Acids
/
Hydrolases
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS