Coxsackie virus B types were discriminated by RT-PCR / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 291-293, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-279550
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.</p><p><b>METHODS</b>A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.</p><p><b>RESULTS</b>This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.</p><p><b>CONCLUSION</b>The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Blood
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Classification
/
DNA Primers
/
Enterovirus B, Human
/
Reverse Transcriptase Polymerase Chain Reaction
/
Diagnosis
/
Enterovirus Infections
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2004
Type:
Article
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