Preliminary study on RT-PCR-ELISA method for virus titer testing of live attenuated hepatitis A vaccine / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 261-264, 2004.
Article
in Zh
| WPRIM
| ID: wpr-279559
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the RT-PCR-ELISA method applied for testing live attenuated hepatitis A vaccine titer.</p><p><b>METHODS</b>A solid phase hybridization-enzyme colorimetric detection method was used for detecting specific nucleic acid. Primer labeled with biotin was used to amplify viral gene fragment, then the product was quickly hybridized with the specific probe covalently coupled on DNA-binding microplate wells. Finally, peroxidase-labeled streptavidin was used in colorimetric detection. The results were judged by reading A value. Eleven batches of live attenuated hepatitis A vaccine titer were tested by this method. The results were compared with that of routine cell culture method (CCID50).</p><p><b>RESULTS</b>The sensitivity was similar to routine cell culture method (P>0.05). This method was convenient, fast and specific.</p><p><b>CONCLUSION</b>CCID50 method may be replaced by the RT-PCR-ELISA method in evaluating the titer of live attenuated hepatitis A vaccine.</p>
Full text:
1
Index:
WPRIM
Main subject:
Quality Control
/
DNA, Viral
/
Enzyme-Linked Immunosorbent Assay
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Vaccines, Attenuated
/
Base Sequence
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Sensitivity and Specificity
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Reverse Transcriptase Polymerase Chain Reaction
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Hepatitis A Vaccines
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Hepatitis A virus
/
Genes, Viral
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2004
Type:
Article