Susceptibility of 15 collections of Aedes albopictus from Guizhou to dengue virus oral infection / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the susceptibility of Aedes albopictus to dengue virus infection.</p><p><b>METHODS</b>Aedes albopictus from 15 collections in Guizhou province were challenged orally with dengue virus 1-4 types, respectively. The total RNA from mosquitos were extracted. The viral NS1 gene fragment was amplified with reverse transcriptase polymerase chain reaction (RT-PCR). Dengue virus in mosquitoes was isolated with C6/36 cells. Then the viral antigen was detected by indirect immunofluorescence assay (IFA). Antigen and nucleic acid of dengue virus from 15 geographic strains of Aedes albopictus orally infected with dengue viruses (DEN-1, DEN-2, DEN-3 and DEN-4) were detected by IFA, RT-PCR and the virus was isolated with C6/36 cells, respectively.</p><p><b>RESULTS</b>The rates of Aedes albopictus orally infected with DEN-1, DEN-2, DEN-3 and DEN-4 were 12/15, 12/15, 8/15 and 13/15, respectively.</p><p><b>CONCLUSIONS</b>Different geographic strains of Aedes albopitus in Guizhou were susceptible to dengue viruses.</p>