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Effects of nuclear factor-kappa B p65 ASODN on transforming growth beta-1 and intercellular adhesion molecule-1 of rat hepatic stellate cells / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 762-766, 2008.
Article in Chinese | WPRIM | ID: wpr-279682
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of nuclear factor (NF)-kappa B p65 ASODN on transforming growth factor beta-1 (TGF beta 1) and intercellular adhesion molecule-1 (ICAM-1) of rat hepatic stellate cells (HSC) and the mechanisms of NF-kappa B p65 ASODN in treating liver fibrosis.</p><p><b>METHODS</b>Type IV collagen enzyme digestion and density centrifugation methods were used to separate rat hepatic stellate cells. NF-kappa B p65 ASODN was manually synthesized and completely phosphorothioate-modified. The changes of TGF beta 1 and ICAM-1 mRNA were detected by RT-PCR and albumen of TGF beta 1 and ICAM-1 were detected by ELISA. The changes of NF-kappa B activity were determined by ELISA.</p><p><b>RESULTS</b>NF-kappa B activity and the expressions of ICAM-1 and TGF beta 1 increased after the HSC were treated by TNF alpha. NF-kappa B activity weakened after being treated with NF-kappa B p65 ASODN (0.001-1.000 micromol/L), P less than 0.05 in a dose dependent manner. Transferring NF-kappa B p65 ASODN (0.001-1.000 micromol/L) also weakened the expression of ICAM-1 and TGF beta 1 mRNA and the protein induced by TNF alpha in HSC. It was also in a dose dependent manner, P less than 0.05.</p><p><b>CONCLUSIONS</b>After transferring NF-kappa B p65 ASODN into HSC, their NF-kappa B activity decreased, and their mRNA and protein expressions of ICAM-1 and TGF beta 1 also decreased. This may serve as a new way in treating hepatic fibrosis.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Cell Line / Oligonucleotides, Antisense / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley / Intercellular Adhesion Molecule-1 / Transcription Factor RelA / Transforming Growth Factor beta1 / Hepatic Stellate Cells / Genetics Limits: Animals Language: Chinese Journal: Chinese Journal of Hepatology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Cell Line / Oligonucleotides, Antisense / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley / Intercellular Adhesion Molecule-1 / Transcription Factor RelA / Transforming Growth Factor beta1 / Hepatic Stellate Cells / Genetics Limits: Animals Language: Chinese Journal: Chinese Journal of Hepatology Year: 2008 Type: Article