Screening and identification of human anti-c-Met Fab from a phage antibody library / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 505-508, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-279757
ABSTRACT
<p><b>OBJECTIVE</b>To screen anti-c-Met Fab from a phage antibody library and identify its binding activity.</p><p><b>METHODS</b>The expression of c-Met of HCC lines was identified by Western blot and immunofluorescence. Antibodies against c-Met were screened with immobilized antigen. After five rounds of panning, 30 randomly selected clones were identified by phage ELISA to select specific clones with high affinity. The positive clones were selected for Fab soluble expression in TOP10F and the binding activities were analysed in HCC lines.</p><p><b>RESULTS</b>c-Met expressed in HCC membrane was confirmed by Western blot and immunofluorescence. A Fab fragment named AM2-26 with fine activity to c-Met was selected. AM2-26 binding specificity was confirmed by IP, FACS and immunofluorescence.</p><p><b>CONCLUSION</b>The anti-c-Met Fab binding to c-Met in HCC provides a promising candidate for the biotherapy of hepatoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Immunoglobulin Variable Region
/
Immunoglobulin Fab Fragments
/
Gene Library
/
Cloning, Molecular
/
Peptide Library
/
Proto-Oncogene Proteins c-met
/
Cell Line, Tumor
/
Allergy and Immunology
/
Antibodies
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2008
Type:
Article
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