The Usefulness of Calretinin Immunohistochemistry for Rectal Suction Biopsy to Diagnose Hirschsprung’s Disease / 소아외과
Journal of the Korean Association of Pediatric Surgeons
;
: 23-28, 2016.
Article
in Korean
| WPRIM
| ID: wpr-27976
ABSTRACT
PURPOSE:
Hirschsprung’s disease (HD) is a congenital intestinal disorder with absence of ganglion cells in the intestinal muscle and submucosa. Diagnosis is based on histopathological study such as H&E, and acetylcholinesterase (AchE) immunohistochemistry. Calretinin immunohistochemistry was introduced as a new diagnostic method against limitations of other staining. The aim of this study is to investigate the usefulness of calretinin immunohistochemistry for the diagnosis of HD compared to H&E and AchE.METHODS:
Ten patients with HD and 22 non-HD patients were included in the study. H&E staining, AchE and calretinin immunohistochemistry were performed in all 32 patients. All slides were evaluated by same single pathologist and the diagnostic value was calculated for each H&E stain, AchE immunohistochemical staining, and calretinin immunohistochemical staining.RESULTS:
Calretinin method had sensitivity of 100% and specificity of 100% for diagnosis of HD. Its diagnostic accuracy was 100%. AchE staining showed 100% of specificity and 80% of sensitivity. Diagnostic accuracy of H&E staining was 56.3%.CONCLUSION:
We concluded that calretinin immunohistochemistry is a very useful and valuable method to diagnosis HD patient.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetylcholinesterase
/
Suction
/
Biopsy
/
Immunohistochemistry
/
Sensitivity and Specificity
/
Ganglion Cysts
/
Diagnosis
/
Calbindin 2
/
Hematoxylin
/
Hirschsprung Disease
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Association of Pediatric Surgeons
Year:
2016
Type:
Article
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