Competitive capacity of HIV-1 strains carrying M184I or Y181I drug-resistant mutations / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1081-1086, 2009.
Article
in English
| WPRIM
| ID: wpr-279780
ABSTRACT
<p><b>BACKGROUND</b>Virus with nucleoside reverse transcriptase inhibitors (NRTIs) or nonnucleoside reverse transcriptase inhibitors (NNRTIs) resistant mutations show different evolution tendencies when the anti-viral therapies are interrupted. Understanding the replication fitness of drug-resistant virus is important for the study of the prevalence of drug-resistance. For this purpose, we characterized the replication capacity of HIV-1 virus carrying lamivudine (3TC) or nevirapine (NVP) resistant mutations.</p><p><b>METHODS</b>3TC and NVP resistant variants were induced in vitro by selecting wild type virus in the presence of drugs. For the competitive replication assay, drug-resistant variants were cocultured with wild-type virus in the presence or absence of drugs. The ratios of the viral species were determined over time by using a real-time RT-PCR-based assay.</p><p><b>RESULTS</b>3TC-resistant (M184I mutation) and NVP-resistant (Y181I mutation) virus should be selected in vitro in two different ways. The competitive replication assay showed that the ratio of virus carrying a M184I mutation increased from 98.8%, while the wild type virus decreased to 1.2% after 4 passages in the presence of 3TC; the percentage of virus carrying the Y181I mutation increased to 90.5%, while wild type virus decreased to 9.5% in the presence of NVP. In the absence of drugs, the ratio of virus carrying the M184I mutation decreased to 5.3%, while wild type virus increased to 94.7%; the ratio of virus carrying Y181I increased to 75%, while wild type virus decreased to 25% after 4 passages.</p><p><b>CONCLUSIONS</b>The NVP-resistant virus is fitter than wild type virus even in the absence of NVP that may be the reason that NNRTIs-resistant virus is spreading quickly.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Virus Replication
/
Cell Line
/
HIV-1
/
Reverse Transcriptase Inhibitors
/
Lamivudine
/
Nevirapine
/
Reverse Transcriptase Polymerase Chain Reaction
/
Drug Resistance, Viral
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2009
Type:
Article
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