Roles of PKCα on the biological functions of T cells / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 1354-1359, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-279911
ABSTRACT
<p><b>OBJECTIVE</b>To study the roles of PKCα on the proliferation, apoptosis, differentiation, cytokine production and inducible regulatory T cell (iTreg) induction of T cells.</p><p><b>METHODS</b>T cells from WT (PKCα⁺/⁺) or PKCα knockout (PKCα⁻/⁻) mice were isolated and cultured in vitro. T cell proliferation and apoptosis were determined using ³H thymidine incorporation and CSFE/Annexin V staining. Cytokines production (IL-2, IL-4, IFN-γ and IL-17) was detected using ELISA. CD4⁺T cells were isolated and cultured in vitro via Th17 or iTreg biased condition. Flow cytometry was used to detect the cell differentiation.</p><p><b>RESULTS</b>The production of IL-2 upon TCR stimulation increased, while the contents of IL-4 and IL-17 decreased in the PKCα⁻/⁻ group compared with the PKCα⁺/⁺ group. The differentiation rate of Th17 cells decreased, while the iTreg production increased in the PKCα⁻/⁻ group compared with the PKCα⁺/⁺ group.</p><p><b>CONCLUSIONS</b>PKC-α is proinflammatory.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Lymphocyte Activation
/
Receptors, Antigen, T-Cell
/
T-Lymphocytes
/
Cell Differentiation
/
Cytokines
/
Allergy and Immunology
/
Protein Kinase C-alpha
/
Th17 Cells
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2015
Type:
Article
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