High resolution DNA typing for HLA-A, B, Cw genes by polymerase chain reaction-sequence specific oligonucleotide probes method in the Shenyang Han population / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 665-667, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-279974
ABSTRACT
<p><b>OBJECTIVE</b>To investigate, at the DNA level, the polymorphism of HLA-A, -B, -Cw genes in the Chinese of Han ethnicity in Shenyang.</p><p><b>METHODS</b>Hybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A, -B and -Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area.</p><p><b>RESULTS</b>The numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium.</p><p><b>CONCLUSION</b>Using molecular method, the present authors have analyzed the characteristic of HLA I distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
HLA-A Antigens
/
HLA-B Antigens
/
HLA-C Antigens
/
Oligonucleotide Probes
/
Linkage Disequilibrium
/
China
/
Polymerase Chain Reaction
/
Alleles
/
Gene Frequency
Type of study:
Prognostic study
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS