Polymorphism of DXYS267 locus and application of its Y-specific single nucleotide substitutions / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 577-579, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-279995
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the polymorphism of DXYS267 locus in China Han population and find the application and characters of its Y-specific single nucleotide substitutions.</p><p><b>METHODS</b>The locus was analyzed by PCR and PAGE in silver-staining. The Y-specific STR was amplified with newly designed primers according to the Y-specific single nucleotide substitutions.</p><p><b>RESULTS</b>Six alleles were detected in Han population in Wuhan. Exact tests demonstrated that genotype frequencies did not deviate from Hardy-Weinberg equilibrium. Heterozygosity of DXYS267 was 0.6706, discrimination power (DP) was 0.8433, and the probability of paternity exclusion (PE) was 0.5957. The Y-specific STR of DXYS267 was successfully amplified with the new primer. The 4 alleles for Y-STR were detected with haplotype diversity (HD) 0.6372.</p><p><b>CONCLUSION</b>The DXYS267 locus is appropriate for individual identification and paternity testing. The new primer is useful for individual and paternity testing involving brothers and mixed stains.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Polymerase Chain Reaction
/
Tandem Repeat Sequences
/
Polymorphism, Single Nucleotide
/
Chromosomes, Human, X
/
Chromosomes, Human, Y
/
Gene Frequency
/
Genetics
/
Genotype
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article
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